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Updated: Feb 15, 2026

Generation of Human Kidney Tubuloids from Tissue and Urine
Published on: April 16, 2021
Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids
Giulia Perticari1, Maroussia M P Ganpat1, Jarno Drost2
1Princess Máxima Center for Pediatric Oncology, Oncode Institute, Heidelberglaan 25, Utrecht 3584 CS, the Netherlands.
Abstract:
Organoids derived from normal human tissue have proven to be effective preclinical models for studying tumor progression through the introduction of driver mutations. In this protocol, we outline techniques for disease modeling using organoids derived from healthy kidney tissue, also known as tubuloids. We provide step-by-step instructions for the establishment and passaging of tubuloids, lentiviral transduction, processing for histological analysis, and preparation for CUT&RUN and single-cell transcriptomic profiling. For complete details on the use and execution of this protocol, please refer to Ganpat et al.1.
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