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Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

Single-cell mass-density measurements using microchannel gradient centrifugation.

Richard Soller¹, Per Augustsson², Rune Barnkob^3,4

¹Lund University, Department of Biomedical Engineering, Lund, 22363, Sweden.

Scientific Reports

|February 13, 2026

Summary

This summary is machine-generated.

We developed a microchannel method for precise single-cell mass density measurement. This technique offers high throughput and affordability, making cell density analysis more accessible.

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Area of Science:

Biophysics
Cell Biology
Microfluidics

Background:

Accurate mass density measurement of single cells is crucial for understanding cellular heterogeneity and function.
Existing methods often lack the throughput or precision required for large-scale analysis.

Purpose of the Study:

To present a microchannel-based adaptation of mass density-gradient centrifugation for precise and high-throughput single-cell mass density measurement.
To introduce methods for microscopic mass density readout and demonstrate the technique's efficacy.

Main Methods:

A workflow involving microchannel filling, centrifugation, and microscopy was employed.
A one-dimensional mass-density gradient was generated instantaneously and repeatably.
Calibration particles and tracer molecules were used for microscopic mass density readout.

Main Results:

The mass density of single yeast cells was successfully measured.
The method achieved a median uncertainty of 3.3% in mass density measurement.
A throughput of approximately 16,000 cells per hour was demonstrated.

Conclusions:

The microchannel-based mass density-gradient centrifugation is a precise, high-throughput, and affordable method for single-cell analysis.
This technique significantly enhances the accessibility of single-cell mass density measurements.
The method's robustness and simplicity make it suitable for widespread adoption in biological research.