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Updated: Feb 15, 2026
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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
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HPLC-MS/MS Method for Monitoring of L-Asparaginase Activity by Using Asparagine and Aspartic Acid Plasma Levels
Cem Kaplan1,2,3, İbrahim Daniş1,3, Durişehvar Özer Ünal1,3
1Faculty of Pharmacy, Department of Analytical Chemistry, İstanbul University, Istanbul, Turkey.
Journal of Mass Spectrometry : JMS
|February 14, 2026
Summary
Understanding L-asparaginase effectiveness in leukemia treatment requires measuring its substrates, L-asparagine and L-glutamine. A validated HPLC-MS/MS method was developed for accurate plasma analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Oncology
Background:
- L-asparaginase is crucial for acute lymphoblastic leukemia (ALL) treatment.
- Understanding enzyme substrate levels (L-asparagine, L-glutamine) is key to optimizing L-asparaginase efficacy.
- Accurate quantification of these substrates in biological matrices is essential.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method.
- To quantify L-asparagine and L-glutamine, the substrates of L-asparaginase, in human plasma.
- To enable better understanding of L-asparaginase effectiveness in acute lymphoblastic leukemia treatment.
Main Methods:
- Developed a novel HPLC-MS/MS method for simultaneous analysis of amino acids.
- Utilized hydrophilic interaction liquid chromatography (HILIC) with gradient elution.
- Optimized mobile phase composition using acetonitrile and ammonium acetate.
Main Results:
- The method demonstrated high selectivity and reproducibility for analyzing L-asparagine, L-glutamine, aspartic acid, and glutamic acid in plasma.
- Established linear ranges for amino acids: 500-5000 ng/mL for asparagine, aspartic acid, glutamic acid; 5-50 μg/mL for glutamine.
- Determined low detection and quantitation limits, indicating high sensitivity.
Conclusions:
- The validated HPLC-MS/MS method is suitable for routine analysis of L-asparaginase substrates in plasma.
- This analytical tool can aid in monitoring L-asparaginase therapy and understanding treatment response in ALL patients.
- Further application of this method can refine therapeutic strategies for acute lymphoblastic leukemia.
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