Optimization of Bioactive Polysaccharide Extraction from Protaetia brevitarsis seulensis Larvae Using Deep Eutectic...

Area of Science:

• Biotechnology
• Natural Product Chemistry
• Food Science

Background:

• Polysaccharides from Protaetia brevitarsis seulensis larvae (PBSL) have potential applications.
• Conventional water extraction (CWE) has limitations in efficiency and purity.

Purpose of the Study:

• To optimize a deep eutectic solvent (DES) method for enhanced polysaccharide extraction from PBSL.
• To compare the yield, purity, and bioactivity of polysaccharides extracted using DES versus CWE.

Main Methods:

• Screening of five choline chloride-based DESs.
• Optimization using response surface methodology (RSM) with a Box-Behnken design.
• Characterization of polysaccharide properties (molecular weight, branching, morphology, stability).
• Assessment of antioxidant and anti-inflammatory activities in vitro.

Main Results:

• 40% aqueous choline chloride:glycerol (1:2) was the optimal DES.
• Optimized DES extraction yielded 18% higher yield and 38% greater purity than CWE.
• DES-extracted polysaccharides had lower molecular weight, higher branching, improved morphology, and better stability.
• DES polysaccharides demonstrated superior antioxidant and anti-inflammatory activities and promoted macrophage activation.

Conclusions:

• DES extraction is a highly efficient method for obtaining high-purity, multifunctional polysaccharides from PBSL.
• The extracted polysaccharides show significant potential for use in functional foods and nutraceuticals.
• DES extraction offers a promising alternative to conventional methods for bioactive polysaccharide isolation.