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Green deep eutectic solvent deproteinization of edible insect polysaccharides: Optimization using artificial neural

Oyindamola Vivian Ojulari1, Ibukunoluwa Fola Olawuyi2, Dongyup Hahn3

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Summary

A novel menthol-based deep eutectic solvent (DES) efficiently purifies insect polysaccharides by removing proteins. This green method preserves polysaccharide structure and shows broad applicability for protein-bound carbohydrate extraction.

Keywords:
Deep eutectic solventGreen purificationInsect-derived polysaccharides

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Area of Science:

  • Biochemistry
  • Green Chemistry
  • Materials Science

Background:

  • Polysaccharide purification from protein-rich sources is hindered by strong carbohydrate-protein interactions.
  • Existing deproteinization methods are often inefficient and solvent-intensive.

Purpose of the Study:

  • To develop a sustainable and efficient deproteinization strategy for insect polysaccharides using deep eutectic solvents (DES).
  • To optimize the DES process and compare its efficacy against conventional methods.

Main Methods:

  • Screening and optimization of menthol-based DES for deproteinization.
  • Application of Response Surface Methodology (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA) for process modeling.
  • Structural and compositional analysis of purified polysaccharides (e.g., FT-IR, NMR, monosaccharide composition).
  • Comparative evaluation with the conventional Sevag method and other insect polysaccharides.

Main Results:

  • The ANN-GA model provided superior prediction accuracy over RSM.
  • Optimal DES conditions yielded high polysaccharide retention (95.30%) and deproteinization (86.95%) rates.
  • DES-purified polysaccharides showed significantly higher sugar and lower protein content compared to Sevag method.
  • DES treatment preserved polysaccharide molecular integrity and backbone structure.
  • The DES method demonstrated broad applicability across multiple insect species.

Conclusions:

  • Menthol-based DES offers a green, efficient, and scalable alternative for polysaccharide deproteinization.
  • This method effectively removes proteins while maintaining polysaccharide quality.
  • The developed DES process has significant potential for purifying various protein-bound polysaccharides.