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Related Experiment Video

Updated: Feb 18, 2026

A High Output Method to Isolate Cerebral Pericytes from Mouse
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A High Output Method to Isolate Cerebral Pericytes from Mouse

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Isolation of Pericytes from Mouse Cortical Tissue Using FACS for Single-cell Sequencing.

Xinyi Cui1, Qingbin Wu1, Shuang Zhang1

  • 1Institute of Microcirculation, Chinese Academy of Medical Sciences & Peking Union Medical College; International Center of Microvascular Medicine, Chinese Academy of Medical Sciences.

Journal of Visualized Experiments : Jove
|February 16, 2026
PubMed
Summary

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This summary is machine-generated.

Researchers developed a new method to isolate mouse brain pericytes for single-cell RNA sequencing. This technique improves cell viability and RNA quality, enabling detailed study of pericyte heterogeneity in neurological disorders.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Genomics

Background:

  • Pericytes are crucial for blood-brain barrier integrity and cerebral blood flow regulation.
  • Pericyte dysfunction is linked to various neurological disorders.
  • Understanding pericyte heterogeneity is key to elucidating their role in neurovascular diseases.

Purpose of the Study:

  • To establish an optimized protocol for isolating mouse brain pericytes for single-cell RNA sequencing (scRNA-seq).
  • To enable high-resolution investigation of pericyte heterogeneity and their mechanisms in neurovascular diseases.

Main Methods:

  • Utilized an enhanced CD13+/CD31- sorting strategy for pericyte isolation.
  • Optimized mechanical and enzymatic dissociation of mouse brain tissue.
  • Introduced a 20% bovine serum albumin (BSA) resuspension step to enhance cell viability and RNA quality for scRNA-seq.

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Related Experiment Videos

Last Updated: Feb 18, 2026

A High Output Method to Isolate Cerebral Pericytes from Mouse
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A High Output Method to Isolate Cerebral Pericytes from Mouse

Published on: January 14, 2020

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Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
10:07

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

Published on: May 26, 2017

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Isolation and Purification of Murine Cardiac Pericytes
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Main Results:

  • Developed a reproducible FACS-based protocol for robust isolation of viable mouse brain pericytes.
  • The optimized method ensures high cell viability and RNA integrity, suitable for scRNA-seq.
  • The protocol effectively isolates pericytes meeting stringent requirements for downstream sequencing applications.

Conclusions:

  • The optimized protocol provides a powerful tool for dissecting pericyte heterogeneity.
  • This method facilitates the study of pericyte roles in the pathogenesis of neurovascular diseases at single-cell resolution.