Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Urinary Tract Infection I: Introduction01:26

Urinary Tract Infection I: Introduction

1.3K
Urinary tract infections (UTIs) impact various parts of the urinary system, including the kidneys, ureters, bladder, and urethra. These infections are generally bacterial, with Escherichia coli being the most common causative agent, often originating from the gastrointestinal tract. However, other bacteria, such as Staphylococcus saprophyticus, Klebsiella pneumoniae, and Proteus mirabilis, are also known to cause UTIs. The type, location, and underlying complexity of the UTI guide both...
1.3K
Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

1.5K
The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
1.5K
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care01:30

Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care

434
A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
434
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

2.9K
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
2.9K
Differential Staining Technique01:26

Differential Staining Technique

2.7K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.7K
Special Staining Techniques01:13

Special Staining Techniques

1.7K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flexible Porous Pomelo Pith Derived Janus Liquid Metal and Adhesive Hydrogel Hybrid Electronic Skins.

Smart medicine·2026
Same author

Myocardial mechanics displaying on visual structural color hydrogel microcolumn arrays.

Journal of nanobiotechnology·2026
Same author

The role of Kiss1 neurons in regulating metabolism and energy balance.

Peptides·2026
Same author

Sodium Ascorbate-Accelerated Gelling Hydrogels With Rapid Self-Mineralized Capacity for Chronic Wounds.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Hierarchical biomimetic scaffold with directional guidance and photothermal properties for the repair of critical-sized bone defects.

Journal of nanobiotechnology·2026
Same author

Recent advances in the stereoselective synthesis of distal biaxially chiral molecules.

Beilstein journal of organic chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
Same journal

Development of a Tapping-Mode Scanning Probe Electrospray Ionization Platform for High-Sensitivity and Long-Term Stability in Single-Cell Mass Spectrometry Imaging of Tissue.

Analytical chemistry·2026
Same journal

A Solid-State Near-Infrared Fluorescent Probe by a Synergistic Extended Conjugated System for Detecting Cys with Long-Term Imaging in Orthotopic Bladder Cancer.

Analytical chemistry·2026
Same journal

Air-Based Porous Array Dielectric Barrier Discharge Ionization Source for Explosive Trace Detection.

Analytical chemistry·2026
Same journal

PSAQ<sup>+1</sup>: Absolute Protein Quantification Using a <sup>13</sup>C<sub>1</sub>-Labeled Protein Standard, Coisolation of Peptide Pairs and LC-PRM.

Analytical chemistry·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 2, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K

Wettable Porous Arrays with Structural Color Barcodes Integration for Multiplex Urinary Tract Infection Bacteria

Chaocheng Feng1,2, Wenzhao Li2, Yu Wang2

  • 1Zhejiang-Finland Joint Laboratory of Gastrointestinal Tumor Metabolism and Nutrition, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.

Analytical Chemistry
|February 17, 2026
PubMed
Summary
This summary is machine-generated.

A new method uses structural color barcodes in porous arrays for rapid, sensitive detection of multiple urinary tract infection (UTI) bacteria. This approach enhances early diagnosis and treatment of common bacterial infections.

More Related Videos

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

21.0K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.5K

Related Experiment Videos

Last Updated: May 2, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

16.7K
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

21.0K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.5K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Medical Microbiology

Background:

  • Urinary tract infections (UTIs) are common and can be severe, necessitating sensitive diagnostic tools.
  • Current multiplex digital nucleic acid amplification technologies (dNAATs) for UTIs are often inefficient.
  • Early and accurate detection of UTI pathogens is critical for patient outcomes.

Purpose of the Study:

  • To develop a novel, highly sensitive, and efficient multiplex detection method for UTI pathogens.
  • To integrate structural color barcodes with superhydrophilic porous arrays for simultaneous target detection.
  • To enable rapid and accurate screening of UTI-causing bacteria.

Main Methods:

  • Development of a superhydrophilic porous array platform with integrated structural color barcodes.
  • Utilizing the distinct reflection wavelengths of color barcodes for multiplex encoding.
  • Employing independent amplification within microwells for absolute nucleic acid quantification.
  • Designing specific probes attached to color barcodes for pathogen identification.

Main Results:

  • The platform demonstrated sensitive detection of single and multiple UTI pathogens.
  • Simultaneous screening of multiplexed UTI bacteria was achieved quickly, accurately, and reproducibly.
  • The method enables absolute quantification of nucleic acids through independent amplification in each unit.
  • The integrated system showed high sensitivity and potential for early infection diagnosis.

Conclusions:

  • The developed porous array platform with structural color barcodes offers a highly sensitive method for UTI bacteria detection.
  • This technology has significant potential for multiplex biomolecule detection and the early diagnosis of infectious diseases.
  • The approach overcomes limitations of traditional dNAATs by enabling efficient, simultaneous multiplex analysis.