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Updated: Feb 19, 2026
Studying Mitochondrial Structure and Function in Drosophila Ovaries
Published on: January 4, 2017
Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes
1Interdisciplinary Genetics and Genomics Program, Iowa State University, Ames, IA 50011, USA; Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA.
Abstract:
Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging. Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes. We describe steps for fly preparation, dissection of abdominal cuticle to expose oenocytes, and mounting. We then detail procedures for time-lapse acquisition of mitochondria labeled with mitoGFP. Optimized imaging parameters enable reproducible visualization of mitochondrial morphology stably for longer durations.
