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Updated: Feb 19, 2026
08:38
Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
Published on: March 19, 2013
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Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction.
Chunyi Pu1, Siyu Liang1, Yue Ma1
1Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, School of Basic Medical Science, Southern Medical University, Guangdong, Guangzhou, 510515, China.
Bioactive Materials
|February 18, 2026
Summary
This study introduces a novel cell sheet therapy using a dynamic, electro-mechanically active substrate for muscle reconstruction. This approach enhances cell density and function, offering a promising new strategy for volumetric muscle loss (VML) repair.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Muscle reconstruction therapies struggle with cell density and functional integration.
- Volumetric muscle loss (VML) presents significant challenges in current regenerative medicine.
Purpose of the Study:
- To develop a pre-priming cell sheet therapy for VML using electro-mechanically bioactive constructs.
- To create a multifunctional platform for scalable, non-invasive cell sheet preparation and transplantation.
Main Methods:
- Fabrication of a near-infrared (NIR)-responsive, wrinkle-patterned, conductive substrate.
- NIR-triggered non-invasive cell sheet harvesting via dynamic wrinkle morphology.
- In vitro electrophysiology, RNA sequencing, and in vivo analysis of VML repair efficacy.
Main Results:
- The microstructured electroactive surface demonstrated superior VML repair compared to cell-suspension therapy.
- Combined electrical and mechanical cues promoted myogenic differentiation, growth, and calcium signaling in myoblasts.
- NIR-triggered release mechanism enabled efficient, non-invasive cell sheet harvesting.
Conclusions:
- Engineering dynamic interfacial dynamics is crucial for advanced cell therapies.
- The developed substrate provides electrical and mechanical priming for enhanced tissue regeneration.
- This dynamic electroactive substrate is promising for regenerating electroexcitable tissues and broader regenerative medicine applications.
