Related Experiment Video
Updated: May 7, 2026
09:26
High-throughput Protein Expression Generator Using a Microfluidic Platform
Published on: August 23, 2012
12.3K
Microfluidic transfection systems
Rashin Mohammadi1, Stavros Stavrakis1, Andew J deMello1
1Institute for Chemical & Bioengineering, ETH Zürich, 8093 Zürich, Switzerland.
Advanced Drug Delivery Reviews
|February 18, 2026
Summary
Microfluidic technologies offer advanced solutions for intracellular delivery, overcoming limitations of traditional transfection methods. These platforms enhance delivery efficiency and cell viability, paving the way for precise and scalable therapeutic manufacturing.
Area of Science:
- Biotechnology
- Cell Biology
- Bioengineering
Background:
- Transfection is crucial for cell engineering, gene function studies, and developing targeted therapies like CAR-T cells.
- Conventional methods (viral, nanoparticle, electroporation) struggle with large cargo delivery, cell viability, and phenotype maintenance.
- Microfluidic technologies offer precise control at the microscale for intracellular delivery applications.
Purpose of the Study:
- To review emerging microfluidic transfection technologies.
- To highlight how microfluidics overcomes limitations of conventional transfection.
- To discuss the role of microfluidics in advancing cell engineering and therapeutic manufacturing.
Main Methods:
- Review of recent advancements in microfluidic electroporation, mechanoporation, and sonoporation.
- Analysis of microfluidic platforms for intracellular delivery.
- Discussion of analytical detection and process automation in microfluidic systems.
Main Results:
- Microfluidic advances improve payload delivery control, enhancing efficiency and cell viability.
- Microfluidic platforms support scalable and high-throughput transfection operations.
- Integration of analytical detection and automation facilitates single-cell studies and manufacturing.
Conclusions:
- Microfluidic technologies represent a significant advancement in intracellular delivery.
- These platforms offer precise, efficient, and scalable solutions for cell engineering and therapeutic development.
- Further development is needed for clinical translation of microfluidic transfection methods.
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