Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Procedure for a normalised thyroxine determination in small sephadex columns.

W T Goedemans, P C Bartels

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Total amount of ECP per eosinophil as indicator for the activity state of eosinophils.

    Scandinavian journal of clinical and laboratory investigation·2001
    Same author

    Reduction of preanalytical errors due to in vitro activation of coagulation.

    Clinical laboratory·2001
    Same author

    Effect of immunotherapy on eosinophil activation in pollen sensitive children.

    Clinical laboratory·2001
    Same author

    Hemostatic imbalance in active and quiescent ulcerative colitis.

    The American journal of gastroenterology·2001
    Same author

    Surplus value of eosinophil count and ECP to diagnose and monitor asthmatic patients.

    The Netherlands journal of medicine·2001
    Same author

    Long-term reduction of plasma homocysteine levels by super-flux dialyzers in hemodialysis patients.

    Kidney international·2001
    Same journal

    Determination of amniotic fluid acetylcholinesterase activity in the antenatal diagnosis of foetal malformations: the first ten years.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Antibodies as a source of analytical errors.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Determination of methotrexate in serum: the aca MTHO immunoassay and HPLC compared.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Improved sample and reagent pipetting with the MINILAB.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Enzyme immunoassay of testosterone using nitrocellulose discs as the solid phase.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Interference of immunoglobulins in the radioimmunoassay of human beta-endorphin.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    See all related articles

    This study presents a rapid, simple test for measuring normalized serum thyroxine (T4) using minimal blood. The method accurately corrects for variable thyroxine binding globulin, ensuring reliable results.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Endocrinology

    Background:

    • Accurate measurement of serum thyroxine (T4) is crucial for diagnosing thyroid disorders.
    • Thyroxine binding globulin (TBG) levels can vary significantly, affecting total T4 measurements.
    • Existing methods may require larger sample volumes or are less adaptable to variable TBG concentrations.

    Purpose of the Study:

    • To develop and validate a rapid, simple, and accurate method for measuring normalized serum thyroxine (T4).
    • To address the challenge of variable thyroxine binding globulin (TBG) concentrations in T4 assays.
    • To minimize the required serum volume for T4 testing.

    Main Methods:

    • Utilized small Sephadex columns for serum thyroxine measurement.
    • Implemented a reintroduction step of test serum during the competitive binding stage.

    Related Experiment Videos

  • Validated the method against the thyroxine concentration and radioactive T3 uptake (T4--RT3U) index and a commercial normalized thyroxine (T4N) assay.

    • Main Results:

    • The developed method provides corrected T4 values, accounting for variable TBG concentrations.
    • Requires only 125 microliters of serum per test.
    • Demonstrated strong correlation with the T4--RT3U index and a commercial T4N assay.
    • Exhibited a day-to-day variation of 6% (CV).

    Conclusions:

    • The described method offers a rapid, simple, and reliable approach for measuring normalized serum thyroxine.
    • Its ability to correct for TBG variability and low serum volume requirement makes it suitable for clinical use.
    • The test provides accurate T4 measurements comparable to established methods.