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Updated: May 3, 2026

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
Published on: February 16, 2018
Few-shot real-time quantitative phase imaging based on lightweight networks
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Quantitative phase imaging plays a crucial role in fields such as environmental monitoring and atmospheric turbulence analysis. However, existing deep learning methods remain hampered by high computational complexity and heavy reliance on large-scale datasets, rendering them unsuitable for millisecond-scale real-time processing in demanding applications. Here, we propose a few-shot real-time quantitative phase imaging algorithm based on a lightweight network. The algorithm introduces what we believe to be a novel StarNet architecture that enables efficient feature extraction and dimensional expansion, significantly reducing computational overhead. Experimental results show that it can still provide phase reconstruction with millisecond-scale each frame, even under conditions of low signal-to-noise ratio and diverse random phase disturbances. This algorithm not only advances the practical deployment of real-time QPI technology but also offers an efficient and reliable solution for multi-frame inverse imaging problems in data-scarce scenarios.
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

