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STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.

Rongxin Zhao1, Kexin Fang2, Xiaofei Zhang3

  • 1Department of Dermatology, Pudong New Area People's Hospital, Shanghai 201200; P.R. China.

Molecular Medicine Reports
|February 20, 2026
PubMed
Summary

Signal transducer and activator of transcription 1 (STAT1) drives melanoma progression by upregulating tubulin β4A (TUBB4A). Targeting this STAT1-TUBB4A axis may offer new melanoma treatment strategies.

Keywords:
STAT1TUBB4Aapoptosiscutaneous melanomamigration

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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Melanoma pathogenesis involves complex signaling pathways.
  • Signal transducer and activator of transcription 1 (STAT1) and tubulin β4A (TUBB4A) are implicated in cancer, but their specific roles in melanoma require elucidation.

Purpose of the Study:

  • To investigate the roles of STAT1 and TUBB4A in melanoma cell proliferation, motility, and apoptosis.
  • To establish foundational evidence for developing precision therapeutic interventions targeting these proteins in melanoma.

Main Methods:

  • Gene silencing of STAT1 and overexpression of TUBB4A in melanoma cell lines (A375, RPMI-7951).
  • In vitro assays: Cell Counting Kit-8, colony formation, Transwell migration, and flow cytometry for apoptosis.
  • In vivo murine xenograft model to assess tumor growth.
  • Western blot analysis for protein expression levels.

Main Results:

  • STAT1 downregulation impaired melanoma cell proliferation and motility, increasing apoptosis.
  • TUBB4A overexpression partially reversed these effects, indicating it mediates STAT1's pro-tumorigenic activity.
  • STAT1 knockdown reduced tumor volume in vivo, with TUBB4A overexpression partially restoring growth.

Conclusions:

  • STAT1 promotes melanoma progression by upregulating TUBB4A as a downstream mediator.
  • The STAT1-TUBB4A regulatory axis represents a potential therapeutic target for melanoma.
  • Novel mechanistic insights into melanoma pathogenesis and potential treatment modalities were provided.