Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Whole-mouse immunolabeling at cellular resolution for comprehensive 3D atlases.

Nature protocols·2026

Same author

Pathogenic role of serpin B3-positive neutrophils in reinforcing thrombus stiffening during ischemic stroke.

Blood·2026

Same author

Cross-kingdom miRNA delivery by Panax notoginseng-derived extracellular-like nanoparticles vesicles restores neuronal function after ischemic injury.

Journal of nanobiotechnology·2026

Same author

Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction.

Brain : a journal of neurology·2026

Same author

IGF2BP1 positively regulates CircOGDH accumulation in hypoxia induced stress granules.

Acta pharmaceutica Sinica. B·2026

Same author

Ultraviolet-tyrosinase cascade caged antisense oligonucleotide for precise treatment of hyperpigmentation.

Journal of controlled release : official journal of the Controlled Release Society·2025

Same journal

Protocol for genomic mapping of chromatin targets using high-throughput CUT&RUN.

STAR protocols·2026

Same journal

A protocol for noninvasive quantification of dietary fat absorption in mice.

STAR protocols·2026

Same journal

Protocol for an AlCl<sub>3</sub>-induced Alzheimer's model in zebrafish larvae with optimized pH and behavioral assessment.

STAR protocols·2026

Same journal

Protocol for live cell barcoding and immunophenotyping of human hematological malignancies using cytometry by time of flight.

STAR protocols·2026

Same journal

Generating drug resistance models in human and murine cancer cell lines and assessing cross-resistance to chemotherapeutics and KRAS inhibitors.

STAR protocols·2026

Same journal

Protocol for simultaneous in vivo two-photon imaging and locomotion quantification during olfactory stimulation and pharmacology in walking Drosophila.

STAR protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 22, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

Protocol for isolating plant-derived extracellular vesicles.

Yunyi Chen¹, Le Xu¹, Yuanyuan Yu²

¹Department of Neurology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, China.

|February 20, 2026

Summary

This summary is machine-generated.

We developed a robust protocol to isolate plant-derived extracellular vesicles (PEVs) from various plant sources. This method ensures high purity and efficient recovery of PEVs for further research.

Keywords:

Biotechnology and bioengineering Cell Biology Plant sciences

More Related Videos

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

Related Experiment Videos

Last Updated: Feb 22, 2026

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Isolation and Characterization of Cyanobacterial Extracellular Vesicles

Published on: February 3, 2022

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration

Published on: February 2, 2024

Area of Science:

Plant biology
Cell biology
Biochemistry

Background:

Plant-derived extracellular vesicles (PEVs) are crucial for intercellular communication.
Existing isolation methods often lack reproducibility and efficiency.

Purpose of the Study:

To establish a comprehensive and reproducible protocol for isolating PEVs from diverse plant species.
To ensure efficient enrichment and purification of PEVs.

Main Methods:

Differential centrifugation
Ultrafiltration
Ultracentrifugation
Sucrose density gradient centrifugation

Main Results:

Successfully isolated and purified PEVs from various plant sources.
Achieved efficient removal of cellular debris.
Characterized PEVs by morphology, size distribution, and zeta potential.

Conclusions:

The developed protocol provides a reliable method for PEV isolation.
This method facilitates further investigation into PEV functions in plants.