The human induced pluripotent stem cell line CTGUi-002A was generated from a Perrault syndrome patient
Shen-Lin Wu1, Yang Li1, Tonghai Dou2
1Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, College of Basic Medical Sciences, China Three Gorges University, Yichang 443002, Hubei, China.
Abstract:
We generated an induced pluripotent stem cell (iPSC) line, designated CTGUi002-A, from peripheral blood mononuclear cells (PBMCs) of a 9-year-old female with Perrault syndrome carrying biallelic TWNK mutations (c.811G>A and c.1163C>T), using Sendai virus-mediated delivery of OCT4, SOX2, KLF4, and c-MYC. The CTGUi002-A iPSC line retained the TWNK mutations and exhibited characteristic iPSC morphology, expressed pluripotency markers, maintained a normal karyotype, and demonstrated trilineage differentiation potential.
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