Protocol for chromatin immunoprecipitation using isolated antheridia of Marchantia polymorpha

Chromatin immunoprecipitation (ChIP) is a fundamental technique used to investigate the binding sites of transcription factors (TFs) and histone modifications throughout the genome. Here, we present the ChIP protocol using the model bryophyte Marchantia polymorpha. We describe steps for collecting antheridia, the ChIP procedure, and validating binding sites of TFs by ChIP-qPCR. This protocol, with modifications, can be applied to ChIP and its associated techniques for other TFs or DNA-binding proteins in other tissues of M. polymorpha.