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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.7K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.2K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.7K
Types Of Column Chromatography01:29

Types Of Column Chromatography

14.0K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
14.0K
Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

2.6K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
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Updated: Feb 24, 2026

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
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Recent advancements in liquid chromatography column technologies: Manufacturing, connecting and parallel using.

Lin Lv1, Hanchen Cao1, Yeming Hu1

  • 1Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361005, China.

Journal of Chromatography. A
|February 22, 2026
PubMed
Summary

Advancements in high-throughput liquid chromatography (HTLC) column technology are crucial for omics and pharmaceutical research. Innovations in column manufacturing, connection, and parallel use boost analytical speed and resolution for applications like drug discovery.

Keywords:
Column technologyHigh throughput analysisInstrumentationLiquid chromatographyPacked column

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Pharmaceutical Science

Background:

  • The rapid growth of omics science and pharmaceutical research necessitates faster analytical methods.
  • High-throughput liquid chromatography (HTLC) is a key technology driven by these demands.
  • Column technology is a critical component for enhancing HTLC system performance, impacting both speed and resolution.

Purpose of the Study:

  • To review recent significant advancements in liquid chromatography column technologies specifically for HTLC applications.
  • To focus on key developments in column manufacturing, connecting strategies, and parallel utilization.
  • To highlight how integrated column advancements support high-throughput analytical fields.

Main Methods:

  • Literature review of recent key advancements in liquid chromatography column technologies.
  • Focus on three core areas: column manufacturing techniques, column connecting methods, and parallel column usage strategies.
  • Analysis of how these advancements contribute to overall HTLC system capabilities.

Main Results:

  • Recent progress in column manufacturing has led to improved column performance and longevity.
  • Innovations in column connecting technologies enable more robust and efficient HTLC systems.
  • Developments in parallel column usage strategies significantly increase analytical throughput.

Conclusions:

  • The integrated development of column manufacturing, connecting, and parallel use is fundamental for advancing HTLC.
  • These advancements provide essential support for demanding high-throughput applications.
  • Key application areas benefiting include single-cell proteomics, drug discovery, and large-scale screening analyses.