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Published on: March 31, 2021
Comprehensive screening strategy for potentially hazardous plant secondary metabolites in honey using natural deep
Yuzhe Yuan1, Guodong Mu2, Haitao Xu2
1College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China; State Key Laboratory of Resource Insects, Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Abstract:
Honey is prone to be contaminated by hazardous plant secondary metabolites (HPSMs) when bees collect nectar or pollen with these substances. The establishment of a screening approach helps control honey quality, ensuring safe consumption. A novel natural deep eutectic solvent (NADES) combined with LC-MS/MS was developed to detect eleven types of HPSMs in honey. Optimum extraction protocol was established through theoretical screening, validation and parameter optimisation. Furthermore, matrix-matched calibration curves of 28 target substances were determined with the LOQs of 0.15-3.63 μg/kg, and the recovery of spiked honey samples ranged from 82.37% to 99.17% with the RSD < 10%. Compared to conventional methods, this approach includes a wide range of compound classes, though its coverage of specific subcategories remains restricted, and it doesn't provide a notable improvement in LODs; while it shows significant advantages in low consumption of organic solvents, simplifying the overall procedure, and enhancing environmental friendliness and safety.
