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Updated: Feb 24, 2026

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
Published on: January 11, 2017
Development of covalent inhibitors for bacterial histidine kinases
Patricia D Rodriguez1, Conrad A Fihn2, Manibarsha Goswami1
1Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota, 55455, USA. carlsone@umn.edu.
Abstract:
Antivirulence therapies offer an alternative strategy against antimicrobial resistance. We synthesized 2-aminobenzothiazole sulfonyl fluoride analogs for covalent histidine kinase inhibition. In vitro and in cellulo assays also identified lead inhibitors for affinity-based probe development. However, alkyne incorporation reduced bioactivity, emphasizing the challenges of designing functional probes while preserving inhibitor potency.
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