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Related Concept Videos

Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

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Multichemical Analytical Strategy for Screening Key Differential Compound between Raw and Processed Viticis fructus.

Zijing Zhang1,2, Wenwen Li2, Ye Shang2

  • 1First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, 300193 Tianjin, China.

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|February 23, 2026
PubMed
Summary

This study identified key chemical differences between raw and processed Viticis fructus (VF) using advanced chromatography and chemometrics. These findings help ensure the quality and effective clinical use of VF.

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Area of Science:

  • Pharmacognosy
  • Analytical Chemistry
  • Natural Product Chemistry

Background:

  • Viticis fructus (VF), derived from Vitex species, is used raw and processed.
  • Understanding processing-induced chemical changes in VF is crucial for its application and quality control.

Purpose of the Study:

  • To identify key differential compounds between raw and processed Viticis fructus.
  • To establish a reliable analytical strategy for distinguishing between raw and processed VF.

Main Methods:

  • High-performance liquid chromatography tandem diode array detector (HPLC-DAD) and ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) were used for chemical fingerprinting.
  • Chemometrics analysis was employed to screen differential compounds.
  • Ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QQQ-MS/MS) was developed for targeted validation of specific compounds.

Main Results:

  • Chemical fingerprints of raw and processed VF were successfully constructed.
  • Twenty-nine differential compounds were initially identified between raw and processed VF.
  • Specific compounds like caffeic acid, succinic acid, and casticin were validated as important markers for distinguishing VF forms.

Conclusions:

  • Processing significantly alters the chemical composition of Viticis fructus.
  • The developed analytical strategy provides a valuable reference for the quality evaluation and rational clinical application of VF.
  • This research clarifies the impact of processing on VF ingredients.