Oligonucleotide Selective Detection by Levitated Optomechanics

This study examines the detection of oligonucleotide-specific signals in sensitive optomechanical experiments. Silica nanoparticles were functionalized using ZnCl₂ and 25-mers of single-stranded deoxyadenosine and deoxythymidine monophosphate which were optically trapped by a 1550 nm wavelength laser in vacuum. In the optical trap, silica nanoparticles behave as harmonic oscillators, and their oscillation frequency and amplitude can be precisely detected by optical interferometry. The data was compared across particle types, revealing differences in frequency, width, and amplitude of peaks with respect to motion of the silica nanoparticles which can be explained by a theoretical model. Data obtained from this platform was analyzed by fitting Lorentzian curves to the spectra. Dimensionality reduction detected differences between the functionalized and nonfunctionalized silica nanoparticles. Random forest modeling provided further evidence that the fitted data were different between the groups. Transmission electron microscopy was carried out but did not reveal any visual differences between the particle types.