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DDX24 modulates angiogenesis by promoting CCR4-NOT complex-dependent mRNA decay.

Simeng He1,2, Bin Li3, Fangbin Chen1,2

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|February 23, 2026
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Summary
This summary is machine-generated.

DEAD-box RNA helicase 24 (DDX24) regulates mRNA stability in endothelial cells, impacting vascular development and angiogenesis. This discovery offers new therapeutic targets for diseases related to blood vessel formation.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DEAD-box (DDX) RNA helicases are crucial for gene regulation and RNA metabolism.
  • Previous research linked DDX24 dysfunction to vascular development issues, but its specific role in angiogenesis was unknown.

Purpose of the Study:

  • To elucidate the precise role of DDX24 in RNA metabolism within the context of angiogenesis.
  • To identify DDX24-bound messenger RNAs (mRNAs) in endothelial cells.

Main Methods:

  • Infrared cross-linking immunoprecipitation sequencing (irCLIP-seq) was employed to identify DDX24-bound mRNAs.
  • Functional assays were performed to assess the impact of DDX24 on endothelial cell functions and mRNA targets.

Main Results:

  • DDX24 directly binds and regulates specific mRNAs, including CLEC14A and ERG, which are vital for vascular development and angiogenesis.
  • DDX24 was found to promote the decay of these target mRNAs.
  • This mRNA decay is dependent on the CCR4-NOT deadenylase complex.

Conclusions:

  • DDX24 plays a significant role in regulating mRNA stability in endothelial cells.
  • DDX24-mediated regulation of mRNA stability is critical for endothelial cell function and angiogenesis.
  • These findings highlight DDX24 as a potential therapeutic target for angiogenesis-related diseases.