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Cells of the Adaptive Immune Response

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

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Related Experiment Video

Updated: May 8, 2026

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

In Vitro Resident Memory CD8 T Cell Differentiation Using Epithelial Organoid-T Cell Co-culture System.

Ying Lin¹, Julian C Ramprashad², Elizabeth L Hillier³

¹Department of Molecular Microbiology and Immunology, Brown University; Pathobiology Graduate Program, Brown University; ying_lin@brown.edu.

Journal of Visualized Experiments : Jove

|February 23, 2026

Summary

This summary is machine-generated.

Researchers developed a new organoid co-culture system to study how vaginal epithelial cells influence CD8 T cell differentiation. This model helps understand how T cells become tissue-resident memory cells, crucial for immunity at barrier sites.

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Published on: June 27, 2025

Area of Science:

Immunology
Cell Biology
Microbiology

Background:

Barrier mucosal tissues are critical sites for T cell differentiation and function.
Epithelial cells within these tissues significantly influence T cell development.
Existing model systems and tools limit the understanding of epithelial-derived signals shaping T cell biology.

Purpose of the Study:

To develop and validate a novel murine vaginal epithelial organoid-CD8 T cell co-culture system.
To model the dynamic interactions between infected epithelial cells and virus-specific CD8 T cells.
To investigate epithelial cues driving CD8 T cell differentiation in a relevant microenvironment.

Main Methods:

Establishment of a murine vaginal epithelial organoid culture.
Co-culture of organoids with virus-specific CD8 T cells.
Phenotypic and functional analysis of T cell responses within a 3D epithelial microenvironment.

Main Results:

Demonstrated the differentiation of effector CD8 T cells into tissue-resident memory T cells (TRM).
Validated the system for analyzing T cell responses in a physiologically relevant context.
Showcased the system's utility in studying epithelial-T cell interactions.

Conclusions:

The developed co-culture system provides a reductionist approach to study epithelial-derived signals.
This model facilitates investigation into molecular mechanisms of CD8 T cell differentiation at barrier organs.
The system aids in understanding the regulation of protective immunity and immunopathology by epithelial cues.