Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Programmable Hierarchical Assembly of Atomically Precise Metal Nanoclusters Using Supra-Amphiphilic Nucleic Acids.

JACS Au·2026
Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026
Same author

Structure-Controlled Molecular Recognition and Charge Transport in Metallized DNA Nanosheets.

Journal of the American Chemical Society·2026
Same author

Author Correction: Atomically precise photothermal nanomachines.

Nature materials·2026
Same author

L-DNA-Based Framework Nucleic Acid Nanodevice for Lysosomal ATP Imaging.

Analytical chemistry·2026
Same author

DNA-Programmed Amorphous PtCu Nanohybrids With Spatially Partitioned Functions for Hydrogen Evolution.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

A predisposing effect of HLA class II genes in celiac disease by skewing the naive CD4<sup>+</sup> T cell receptor repertoire.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Wave propagation in fluid-saturated nanoporous media: Upscaling molecular mechanics into continuum-level description.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Collagen-producing eye cell atlas reveals distinct fibroblast fates in early injury vs. fibrotic subretinal disease.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Knotted solid tori in contact manifolds.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Biophysical fitness landscape design traps viral evolution.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Cryo-EM of the eukaryotic purine transporter UapA demonstrates intramolecular and lipid regulation of transport.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Feb 25, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

316

Reactive oxygen species-resistant ultrastable super-resolution DNA framework dots.

Chengpin Liang1, Qingting Li1,2, Bin Chen1

  • 1State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Proceedings of the National Academy of Sciences of the United States of America
|February 23, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed photobleaching-resistant DNA framework (SDF) dots that mimic the natural green fluorescent protein (GFP). These ultrastable SDF dots enhance fluorophore photostability for advanced super-resolution imaging applications.

Keywords:
DNA frameworkfluorescent barcodesreactive oxygen species resistancesuper-resolution imagingultra-photostability

More Related Videos

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.5K
Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

Published on: September 24, 2015

10.6K

Related Experiment Videos

Last Updated: Feb 25, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

316
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.5K
Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging ESI

Published on: September 24, 2015

10.6K

Area of Science:

  • Biophysics and biomedical research
  • Materials science
  • Nanotechnology

Background:

  • Nanoconfinement in natural and artificial systems modulates molecular properties for diverse applications.
  • Green fluorescent protein (GFP) utilizes a peptide scaffold for chromophore stabilization via confinement.
  • Developing artificial systems for controlled molecular confinement is crucial for advanced optical applications.

Purpose of the Study:

  • To engineer photobleaching-resistant super-resolution DNA framework (SDF) dots for programmable fluorophore confinement, inspired by GFP.
  • To enhance fluorophore photostability and enable advanced super-resolution microscopy techniques.
  • To create a versatile platform for developing ultrastable fluorescent probes.

Main Methods:

  • Development of DNA framework (SDF) dots with an internal cavity for fluorophore encapsulation.
  • Assessment of photobleaching resistance against reactive oxygen species.
  • Quantification of photostability enhancement with varying fluorophore labeling strategies within SDF dots.
  • Application of SDF dots in super-resolution imaging techniques like stimulated emission depletion (STED) and structured illumination microscopy (SIM).

Main Results:

  • SDF dots exhibit significant resistance to reactive oxygen species-induced photobleaching due to DNA framework shielding.
  • Fluorophore encapsulation within SDF dots resulted in up to ~1.8-fold and ~50-fold enhancement in photostability compared to corner labeling and single labeling on DNA, respectively.
  • Successful STED imaging of live cell membranes for over 30 minutes was achieved.
  • Development of ultrastable super-resolution SIM barcodes capable of distinguishing eighteen colors with ~70 nm spatial resolution.

Conclusions:

  • SDF dots provide a robust and adaptable platform for engineering ultrastable fluorescent probes.
  • The developed nanoconfinement strategy significantly enhances fluorophore photostability for super-resolution imaging.
  • This approach offers a versatile tool for advancing single-particle tracking and imaging in biophysics and biomedical research.