Label-free saliva screening platform using M13 bacteriophage-based 3D plasmonic structures for MRONJ diagnosis
You Hwan Kim1,2, Jin-Ju Kwon3, Minsu Jang1
1Department of Nano Fusion Technology, Pusan National University, Busan, 46241, Republic of Korea.
Abstract:
Medication-related osteonecrosis of the jaw (MRONJ) is a severe complication associated with antiresorptive or antiangiogenic agents, often leading to pain, infection, and reduced quality of life. Current imaging-based diagnostics have limitations in detecting lesions smaller than 10 mm. In this study, we propose a label-free saliva screening approach for MRONJ diagnosis using a three-dimensional plasmonic structure based on M13 bacteriophage. Raman spectroscopy was employed to detect metabolite alterations in saliva, which are known to be associated with MRONJ. The M13 bacteriophage facilitates controlled interparticle gap of gold nanoparticles, thereby increasing hotspot density and enhancing Raman signal intensity. Data preprocessing was conducted on saliva Raman spectra collected from MRONJ patients and controls. To filter outliers, we computed Pearson correlation coefficients between each spectra and the group mean and excluded those with coefficients lower than 0.9. A total of 90 spectra were classified using an optimized multi-layer perceptron model, yielding a specificity of 84.6%, sensitivity of 100.0%, and an AUC of 0.92. This study demonstrates the potential of a saliva-based, non-invasive MRONJ screening strategy. Subsequent research should expand clinical datasets and investigate broader diagnostic applications.
Insights
A novel saliva test using M13 bacteriophage and Raman spectroscopy shows promise for early detection of medication-related osteonecrosis of the jaw (MRONJ). This non-invasive approach could improve diagnosis for patients on antiresorptive or antiangiogenic therapies.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Oral Medicine
Background:
- Medication-related osteonecrosis of the jaw (MRONJ) is a serious complication of antiresorptive/antiangiogenic drugs.
- Current diagnostic methods struggle with early detection of small lesions (<10 mm).
- Non-invasive diagnostic tools are needed for timely MRONJ intervention.
Purpose of the Study:
- To develop a label-free saliva screening method for MRONJ diagnosis.
- To utilize M13 bacteriophage-based plasmonic structures for enhanced Raman spectroscopy.
- To assess the diagnostic performance of the proposed saliva-based approach.
Main Methods:
- Fabrication of a 3D plasmonic structure using M13 bacteriophage and gold nanoparticles.
- Raman spectroscopy analysis of saliva metabolites from MRONJ patients and controls.
- Data preprocessing including outlier filtering and classification using a multi-layer perceptron model.
Main Results:
- The M13 bacteriophage structure enhanced Raman signal intensity by increasing hotspot density.
- The optimized model achieved 84.6% specificity and 100.0% sensitivity.
- An area under the curve (AUC) of 0.92 indicated high diagnostic accuracy.
Conclusions:
- A saliva-based, non-invasive screening strategy for MRONJ is feasible.
- This approach demonstrates potential for early MRONJ detection.
- Further studies with larger datasets are warranted to validate and expand diagnostic applications.
More Related Videos
08:50Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
Published on: October 10, 2013
09:04Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
