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Updated: Feb 25, 2026
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Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
Published on: February 25, 2016
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Cyclin D1 demonstrates superior sensitivity and consistent nuclear expression compared with MiTF in cellular
Jeffrey A Sanford1, Damien Abreu2, Jaroslaw J Jedrych2
1Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. jsanfo15@jhmi.edu.
Virchows Archiv : an International Journal of Pathology
|February 23, 2026
Summary
Cyclin D1 is a more sensitive and reliable marker for cellular neurothekeoma than MiTF. This study found consistent, strong nuclear expression of cyclin D1 in all cases, aiding in diagnosis.
Area of Science:
- Dermatopathology
- Oncology
- Molecular Pathology
Background:
- Cellular neurothekeoma is a rare skin tumor with overlapping features with other neoplasms.
- Current diagnostic markers like MiTF have limitations due to inconsistent expression.
- Cyclin D1, a nuclear marker, has not been evaluated for cellular neurothekeoma.
Purpose of the Study:
- To assess the diagnostic utility of cyclin D1 immunohistochemistry in cellular neurothekeoma.
- To compare the sensitivity and expression patterns of cyclin D1 with MiTF.
Main Methods:
- Retrospective analysis of 11 cellular neurothekeoma cases.
- Immunohistochemistry performed for cyclin D1 and MiTF.
- Quantitative scoring using H-score and assessment of high-intensity staining proportion.
Main Results:
- Cyclin D1 showed strong, uniform nuclear expression in 100% of cases (median H-score 205).
- MiTF expression was variable and focal, with a median H-score of 40 and positivity in only 45% of cases.
- Cyclin D1 staining intensity (median 90% of cells) was significantly higher than MiTF (median 10% of cells).
Conclusions:
- Cyclin D1 is a highly sensitive and dependable immunohistochemical marker for cellular neurothekeoma.
- Cyclin D1 offers superior diagnostic performance compared to MiTF in identifying this neoplasm.
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