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Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining
Isabel San Martín Molina1, Hanneke Okkenhaug1, Simon Walker1
1Imaging Facility, Babraham Institute, Babraham Research Campus, Cambridge, UK.
European Journal of Immunology
|February 24, 2026
Abstract:
We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.
Keywords:
B cellsMACSima imaging cyclic stainingT follicular helper cellsfollicular dendritic cellsgerminal centers
