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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Feb 26, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Acoustic Imaging Cytometry for High-Throughput Cell Analysis.

Marc Sorigue¹, Rebeca Jurado¹, Michael D Ward²

¹Functional Cytomics Lab, Germans Trias i Pujol Research Institute (IGTP), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Barcelona, Spain.

Current Protocols

|February 24, 2026

Summary

This summary is machine-generated.

Acoustic imaging cytometry combines flow cytometry with high-resolution imaging for detailed cell analysis. This advanced technique provides both quantitative and morphological data, transforming clinical research in oncology and immunology.

Keywords:

acoustic imaging cytometry brightfield imaging cell morphology analysis clinical research high‐throughput rare cells

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Last Updated: Feb 26, 2026

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Area of Science:

Biotechnology
Cell Biology
Medical Imaging

Background:

Traditional flow cytometry provides quantitative data but lacks morphological detail.
High-resolution imaging offers morphological insights but often at lower throughput.
A gap exists in simultaneously acquiring high-throughput quantitative and detailed morphological cell data.

Purpose of the Study:

To introduce and detail the capabilities of acoustic imaging cytometry.
To demonstrate its application in comprehensive cellular analysis.
To highlight its potential impact on clinical research.

Main Methods:

Integration of high-throughput flow cytometry with high-resolution brightfield imaging.
Utilizing acoustic focusing for precise cell alignment in a fluidic channel.
Capturing uncolored, high-contrast images at rates up to 6000 events/second.

Main Results:

Simultaneous quantitative (cell counts, fluorescence) and morphological (size, shape, internal structures) cell analysis.
High-quality imaging revealing detailed cellular morphology.
Successful application in diverse clinical research areas like oncology, hematology, and immunology.

Conclusions:

Acoustic imaging cytometry offers a transformative dual-modality approach for cell analysis.
This technology provides a comprehensive view of heterogeneous cell populations.
It holds significant promise for advancing clinical research and diagnostics.