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Updated: Feb 26, 2026

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
Adipose tissue expression of Notch signaling genes in relation to insulin sensitivity and obesity in humans
Monika Karczewska-Kupczewska1, Natalia Matulewicz2, Magdalena Stefanowicz2
1Department of Internal Medicine and Metabolic Diseases, Medical University of Bialystok, M.C. Skłodowskiej 24a, 15-276 Białystok, Poland; Department of Metabolic Diseases, Medical University of Białystok, Waszyngtona 15B, 15-269 Białystok, Poland.
Aims:
Notch signaling may regulate adipogenesis. The objective of the present study was to analyze the role of the Notch pathway in the development of insulin resistance and metabolic complications of obesity. We analyzed subcutaneous (SAT) and visceral adipose tissue (VAT) expression of Notch genes in relation to insulin sensitivity, obesity, and metabolic syndrome (MS).
Methods:
Study group 1 comprised 48 individuals with normal-weight, 28 with overweight and 12 with obesity. Group 2 comprised 27 participants with overweight/obesity examined before and after weight loss. Group 3 comprised 8 individuals without obesity, 23 with obesity without MS, 29 with obesity with MS. SAT biopsy was performed in group 1 and 2. In group 3, SAT and VAT were collected during surgery.
Results:
In group 1, SAT DLL1, NOTCH2, NOTCH4, and HEY1 were lower in individuals with obesity compared to normal-weight individuals. In group 2, weight loss increased DLL1, JAG1, NOTCH2, NOTCH4, and HES1. In group 3, both groups with obesity had lower SAT and VAT JAG1, NOTCH2, NOTCH4, and HEY1. Positive correlations between Notch genes and adipogenic genes were observed in all study groups.
Conclusions:
Notch signaling pathway may be associated with healthy adipose tissue through regulation of adipogenesis in humans.
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