Genome characterization of a novel chrysovirus infecting Aspergillus Niger
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Updated: Feb 26, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Li Wang1,2, Futao Huang1,2, Jia Mi3
1Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education (Guizhou Medical University), Guiyang, 550004, China.
Abstract:
We found a double-stranded RNA (dsRNA) mycovirus from Aspergillus niger strain TR-5, which we named Aspergillus niger chrysovirus 1 (AnCV1). The genome of AnCV1 was found to contain four dsRNA segments (dsRNA1-4 from largest to smallest) with lengths of 3,658 bp, 2,132 bp, 2,618 bp, and 2.533 bp. Sequence analysis showed that the viral RdRp (RNA-dependent RNA polymerase) was encoded by dsRNA1, the viral CP (coat protein) was encoded by dsRNA3, and both dsRNA2 and dsRNA4 contained ORFs (open reading frames) encoding hypothetical proteins. Phylogenetic analysis showed that AnCV1 is a new member of the genus Betachrysovirus in the family Chrysoviridae. This is the first report of the complete genome sequence of a chrysovirus infecting A. niger.
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