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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: Jul 3, 2026

Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging

Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging

Published on: July 13, 2010

A practical guideline for in vivo bioluminescence imaging.

Hannah Lee¹, Ji Soo Chae², Hyun Woo Park¹

¹Department of Biochemistry, College of Life Science and Biotechnology, Brain Korea 21 Four Project, Yonsei University, Seoul, Korea.

Molecules and Cells

|February 25, 2026

Summary

This summary is machine-generated.

Bioluminescence imaging (BLI) offers real-time, non-invasive monitoring of cancer in vivo. This guide details BLI implementation, troubleshooting, and data analysis for preclinical tumor research.

Keywords:

Bioluminescence Imaging In-Vivo Imaging System Luciferin Tumor

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Area of Science:

Biomedical imaging
Cancer research
Preclinical models

Background:

Bioluminescence imaging (BLI) is crucial for in vivo cancer studies.
It enables real-time monitoring of tumor progression and treatment efficacy.
Luciferase-expressing cancer cells are commonly used in BLI.

Purpose of the Study:

To provide a comprehensive guide for effective Bioluminescence imaging (BLI) implementation.
To address key considerations such as substrate administration and data quantification.
To offer practical troubleshooting strategies for common BLI challenges in preclinical research.

Main Methods:

Summarizing best practices for luciferin administration.
Detailing methods for accurate BLI data quantification.
Compiling common technical challenges and their solutions.

Main Results:

Effective BLI requires careful attention to substrate delivery and signal measurement.
Standardized protocols enhance the reliability of in vivo cancer monitoring.
Troubleshooting common issues improves the utility of BLI in research.

Conclusions:

Optimized BLI protocols are essential for accurate preclinical cancer research.
This summary serves as a practical resource for researchers using BLI.
BLI remains a powerful tool for in vivo tumor studies when implemented correctly.