A practical guideline for in vivo bioluminescence imaging
Hannah Lee1, Ji Soo Chae2, Hyun Woo Park1
1Department of Biochemistry, College of Life Science and Biotechnology, Brain Korea 21 Four Project, Yonsei University, Seoul, Korea.
Molecules and Cells
|February 25, 2026
Abstract:
Bioluminescence imaging (BLI) using luciferase-expressing cancer cells is a widely adopted method for noninvasive, real-time monitoring of tumor growth, metastasis, and therapeutic responses in vivo. Here, we summarize key considerations for effective implementation of BLI, including luciferin administration and data quantification. We also address common technical challenges and provide practical troubleshooting strategies to support the use of BLI in preclinical tumor models.


