A modified milk ring test for detecting Brucella agglutinins in bulk tank coolers

The Milk Ring Test (MRT) can be used to detect Brucella antibodies in tank milk if the following points are considered: (1) Before testing, the milk must be stored at least 72 hours at 6 degrees C. (2) To obtain a reliable reaction one must use 8 ml milk and 0.8 ml antigen, instead of 1 ml milk and 0,05 ml antigen. (3) The 4 vol.% antigen appears to be more sensitive than the 5 vol.% antigen. (4) A standard negative milk sample should be included in a batch to be examined. It can help to determine doubtful results. (5) For further determination of a doubtful result obtained from the 1 ml milk reaction, 3 ml milk and 0.05 ml antigen can be used.