Highly fluorescent method for ultra-sensitive estimation of delafloxacin in human plasma: greenness assessment

A simple, rapid, sensitive, and reliable spectrofluorimetric approach has been developed for the analysis of delafloxacin (DEL) in human plasma. The proposed method is based on the formation of a micellar fluorescent complex through the interaction of DEL with 2% sodium dodecyl sulfate (SDS) using a phosphate buffer (pH 4.2), producing a pronounced emission at 470 nm upon excitation at 380 nm. The experimental conditions that affected the one-pot synthesis were investigated and optimized. The calibration curve exhibited a linear relationship over a concentration range of 2.0-120.0 ng mL^-1, with detection (LOD) and quantitation limits (LOQ) 0.6 and 1.9 ng mL^-1, respectively. The designed approach was validated following the ICH and FDA guidelines, producing a precise analysis of DEL in its tablets and plasma samples with high recovery percentage. The results provide that the method possesses excellent eco-friendly properties, highlighting its strong compatibility with the principles of green chemistry.