Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advances in Differentiation of Induced Pluripotent Stem Cell-Derived Corneal Endothelial Cells: Pathway Insights and Evaluation of Characterization Practices.

The American journal of pathology·2026
Same author

Reducing the Carbon Footprint of Refractive Surgery Through Same-Day Postoperative Care.

Journal of cataract and refractive surgery·2026
Same author

Efficacy and Safety Assessment of 5-Fluorouracil, Irinotecan and Oxaliplatin-Loaded Implants in Mouse and Pig Models for Pancreatic Cancer Therapy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Additive-Free Edge-Functionalized Graphene Dough.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Corrigendum to "Ulvan structural modification enhances stability and cell compatibility of GelMa based bioinks for tissue engineering" [Int. J. Biol. Macromol. 321 (2025) 146461].

International journal of biological macromolecules·2026
Same author

Determinants of Intergrader Agreement for Key Retinal Photography and OCT Biomarkers in AMD.

Translational vision science & technology·2025
Same journal

Hydrogel-Encapsulated Primed MSCs Enhance Regeneration in Full-Thickness Porcine Burn Wounds.

Tissue engineering. Part A·2026
Same journal

Unidirectional Porous Carbonate Apatite Fabricated by Gelatin-Based Freeze Casting for Bone Regeneration.

Tissue engineering. Part A·2026
Same journal

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026
Same journal

Impact of IFN-γ-Pretreated Umbilical Cord Mesenchymal Stem Cells Implanted in Mesh on Pelvic Organ Prolapse.

Tissue engineering. Part A·2026
Same journal

The Driving Force of Hierarchical Collagen Fiber Formation: A Review of Tendon, Ligament, and Meniscus Mechanobiology.

Tissue engineering. Part A·2026
Same journal

A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.

Tissue engineering. Part A·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

17.4K

A New Bioprinted Dual-Layered Corneal Structure Using Collagen-Based Bioinks.

Huasheng Huang1,2, Yunong Yuan1,3, Yuan Fang1,2

  • 1School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia.

Tissue Engineering. Part A
|February 26, 2026
PubMed
Summary
This summary is machine-generated.

Researchers created a novel, curved, dual-layer bioprinted corneal model using specific collagen bioinks. This advanced tissue engineering platform enhances the study of corneal stromal-endothelial interactions and graft integration.

Keywords:
3D bioprintingcollagencorneatissue engineering

More Related Videos

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
09:03

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Published on: January 3, 2018

14.0K
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

12.9K

Related Experiment Videos

Last Updated: Feb 28, 2026

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
08:34

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

17.4K
Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
09:03

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Published on: January 3, 2018

14.0K
Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

12.9K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Ophthalmology

Background:

  • Current bioengineered corneal models often lack anatomical curvature and native extracellular matrix (ECM) composition.
  • This limits their utility for studying critical aspects like graft integration and stromal-endothelial cell interactions.

Purpose of the Study:

  • To develop a physiologically relevant, anatomically curved, dual-layer bioengineered corneal construct.
  • To create a platform for studying corneal stromal-endothelial interactions and evaluating graft integration.

Main Methods:

  • Developed a bioprinted, dual-layer corneal model using corneal stromal cells in type I collagen (Col-I) and corneal endothelial cells on collagen type IV (Col-IV).
  • Utilized a curved support to replicate native corneal curvature and employed ECM-specific, human-derived collagen bioinks.
  • Assessed cell viability, endothelial layer formation, histological and immunofluorescence characteristics, curvature retention, transparency, and interfacial integrity over 3 weeks.

Main Results:

  • Achieved high cell viability (>90%) and formation of a continuous endothelial layer.
  • Confirmed distinct layering, appropriate cellular morphology, and correct phenotypic marker expression for both cell types.
  • The construct maintained curvature, transparency, and integrity for 3 weeks and adhered to ex vivo corneal tissue.

Conclusions:

  • The developed bioprinted corneal construct is anatomically curved and multilayered, offering a physiologically relevant in vitro model.
  • This model provides a valuable platform for advancing corneal tissue engineering research, particularly for investigating stromal-endothelial architecture and cell interactions.