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Updated: Feb 28, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Published on: September 25, 2019
Generation and characterization of SOX17-specific EGFP expressing human induced pluripotent stem cell line,
Dae Hoon Yoo1, Delger Bayarsaikhan2, Jaewon Lee2
1Division of Intractable Disease Research, Korea National Institute of Health, Osong, Cheongju, the Republic of Korea.
Abstract:
We generated a human induced pluripotent stem cell (hiPSC) reporter line in which EGFP was inserted in-frame at the C-terminus of the endogenous SOX17 locus using CRISPR/Cas9-mediated homologous recombination. The targeted clone, KSCBi017-A-4, was isolated by puromycin selection and validated by PCR and Sanger sequencing. This SOX17-EGFP hiPSC line retains a normal karyotype and pluripotency and displays specific EGFP expression upon directed definitive endoderm differentiation. This reporter line provides a reliable tool for monitoring SOX17 expression during human endoderm specification.
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