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NMR Profiling of Milk from Treated Dried off Cows
Antonella Caterina Boccia1, Laura Ruth Cagliani1, Dalila Iannone1
1National Research Council, Institute of Chemical Sciences and Technologies "G. Natta" (SCITEC), via A. Corti 12, 20133 Milan, Italy.
None:
The milk metabolite profiles of dairy cows during the dry-off and peripartum periods were investigated using 1H NMR combined with chemometric analysis to evaluate the effects of different dry-off management strategies. Milk samples were collected 14 days before dry-off (T0) and 28 days after calving (T1) from cows receiving an internal teat sealant combined with intramammary antibiotics (CTR), an internal teat sealant alone (SIG), or an internal teat sealant associated with dietary supplementation of lyophilized Aloe arborescens (ASIG). Analysis of both aqueous and organic milk extracts revealed no significant metabolite differences among treatment groups. In contrast, a clear discrimination was detected between samples collected at T0 and T1. Aqueous extracts at T0 were characterized by higher levels of choline, butyrate, branched-chain amino acids, and N-acetylated compounds, whereas T1 samples exhibited higher levels of saccharides, citrate, phosphorylcholine, and galactose-1-phosphate. Organic extracts at T0 showed higher concentrations of conjugated linoleic acids (CLAs) and caproleic acid. These findings indicated that the physiological stage of the cows had a more pronounced impact on milk metabolite composition than the dry-off treatments, with no detrimental effects on milk composition or overall metabolite balance.
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