Meditation in Motion: Sport Type and Meditation Level Shape Gut Microbiota Profiles in Aikido and Tai Chi...

Mind-body practices integrating movement and meditation, such as Tai Chi and Aikido, have been proposed to influence the gut-brain axis through combined physiological and psychological pathways. However, evidence regarding their association with gut microbiota composition remains limited. This study explored gut microbiota diversity and taxonomic profiles in regular practitioners of Tai Chi and Aikido across different levels of meditation depth. Forty-two adults practicing Tai Chi or Aikido provided fecal samples for 16S rRNA sequencing, and meditation depth was assessed using the Meditation Depth Questionnaire (MEDEQ). Alpha diversity did not differ significantly between groups, although a descriptive trend toward higher diversity with increasing meditation depth was observed. Beta-diversity analyses suggested compositional differences associated with meditation level (ANOSIM R = 0.191, p = 0.035), along with an exploratory interaction signal between practice type and meditation depth (ANOSIM R = 0.296, p = 0.001). Taxonomic profiling highlighted distinct microbial patterns associated with both practice type and meditation depth. Short-chain fatty acid-associated genera, including Faecalibacterium and Roseburia, were relatively more abundant in Aikido practitioners with higher meditation scores, whereas Tai Chi practitioners showed higher relative abundances of Prevotella and Collinsella. Overall, these findings indicate that meditative movement practices are associated with distinct gut microbiota compositional patterns within this cohort. Given the exploratory and cross-sectional design, these results should be interpreted as hypothesis-generating. Future longitudinal studies incorporating functional and clinical outcomes are needed to clarify underlying mechanisms.