Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane bridges and nanodomain partitioning govern membrane protein targeting to lipid droplets.

Nature cell biology·2026
Same author

Excited-State Pathway Switching via Reversible Structural Phase Transitions in Sb<sup>3+</sup>-Doped Cadmium Halides.

Angewandte Chemie (International ed. in English)·2026
Same author

Dimensionality-dependent electronic and vibrational dynamics in low-dimensional organic-inorganic tin halides.

Nature communications·2026
Same author

Highly coherent organic lasing based on etch-free metasurface.

Nature communications·2025
Same author

Polymer-Functionalized Liposomes as Universal Nanocarriers for Drug Delivery: Single Particle Insights on Size-Dependent Performance and Intracellular Behavior.

ACS applied materials & interfaces·2025
Same author

Chromatin boundary permeability is controlled by CTCF conformational ensembles.

bioRxiv : the preprint server for biology·2025
Same journal

Scalable Synthesis of Calcium Fluoride Nanoparticles as a Novel Ultrasound Contrast Agent for Imaging Tumor Targeted Delivery of Therapeutics.

Chemical & biomedical imaging·2026
Same journal

Bipolar Electrochemiluminescence at Single Gold Microbeads Trapped by Micropipettes.

Chemical & biomedical imaging·2026
Same journal

Multiplexed Detection of Reactive Biomolecules via Chemoresponsive DNA Accumulation on Fluorescence-Encoded Beads.

Chemical & biomedical imaging·2026
Same journal

Peptide-Based pH-Responsive MRI-CEST Agents: In Vivo Comparison between a Selected Pentapeptide and the Established Iopamidol Reference in Tumor pH Mapping Ability.

Chemical & biomedical imaging·2026
Same journal

Red Blood Cell-Encapsulated Nanoparticles for Long-Circulating, Improved Specificity Functional MRI.

Chemical & biomedical imaging·2026
Same journal

Bioimaging of Metals.

Chemical & biomedical imaging·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.3K

Seeing the Unseen: Super-Resolution Microscopy in Protein Aggregation Research.

Molly J M Turner1,2, Junsheng Chen1,2, Nikos S Hatzakis1,2,3,4

  • 1Department of Chemistry, Faculty of Science, University of Copenhagen, Copenhagen 2100, Denmark.

Chemical & Biomedical Imaging
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

Super-resolution microscopy visualizes biomolecular structures, aiding neurodegenerative disease research. Techniques like STED, SIM, and SMLM offer detailed insights into protein aggregation mechanisms and pathology.

Keywords:
aggregation pathwaysdistributionmorphologyneurodegenerative diseasepathologyprotein aggregationsuper-resolution microscopytoxicity

More Related Videos

4D Imaging of Protein Aggregation in Live Cells
08:59

4D Imaging of Protein Aggregation in Live Cells

Published on: April 5, 2013

17.8K
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.2K

Related Experiment Videos

Last Updated: Feb 28, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.3K
4D Imaging of Protein Aggregation in Live Cells
08:59

4D Imaging of Protein Aggregation in Live Cells

Published on: April 5, 2013

17.8K
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.2K

Area of Science:

  • Biophysics
  • Cell Biology
  • Neuroscience

Background:

  • Super-resolution microscopy overcomes diffraction limits for detailed biomolecular visualization.
  • Protein aggregation is central to neurodegenerative diseases like Alzheimer's and Parkinson's.

Purpose of the Study:

  • To review super-resolution microscopy techniques for studying protein aggregation.
  • To compare STED, SIM, and SMLM for protein aggregation analysis.
  • To highlight applications in understanding disease pathology.

Main Methods:

  • Principles of Stimulated Emission Depletion (STED) microscopy.
  • Principles of Structured Illumination Microscopy (SIM).
  • Principles of Single-Molecule Localization Microscopy (SMLM).

Main Results:

  • Comparison of STED, SIM, and SMLM strengths and limitations for protein aggregation studies.
  • Overview of recent applications in visualizing aggregate morphology and dynamics.
  • Insights into protein aggregation interactions with cellular components.

Conclusions:

  • Super-resolution microscopy provides crucial insights into protein aggregation in neurodegenerative diseases.
  • STED, SIM, and SMLM are powerful tools for dissecting aggregation mechanisms.
  • Further applications promise advancements in understanding disease pathology.