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Analyte Adsorption and Distribution01:09

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Intelligent Cellulose-Based Sorbent for Light-Driven Solid-Phase Extraction of Amphetamine.

Yuhong Xie1, Rui Zhang1, Yifan Liu1

  • 1College of Environment & Safety Engineering, Fuzhou University, Fuzhou, Fujian 350108, China.

Langmuir : the ACS Journal of Surfaces and Colloids
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

A novel cellulose-based sorbent (Cell-Azo) enables light-controlled solid-phase extraction of amphetamine (AMP). This intelligent material offers efficient, green, and controllable trace AMP analysis in complex samples like urine.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Polymer Chemistry

Background:

  • Developing selective and efficient sorbent materials is crucial for trace analyte extraction.
  • Light-responsive materials offer novel mechanisms for controlled capture and release.
  • Existing methods for amphetamine analysis can be complex and time-consuming.

Purpose of the Study:

  • To develop a light-responsive cellulose-based sorbent (Cell-Azo) for amphetamine (AMP) solid-phase extraction.
  • To investigate the adsorption and light-triggered desorption characteristics of Cell-Azo.
  • To establish a sensitive analytical method for trace AMP detection in complex matrices.

Main Methods:

  • Grafting azobenzene onto cellulose via atom-transfer radical polymerization to create Cell-Azo.
  • Characterization using FT-IR, XPS, and UV-vis spectroscopy.
  • Batch adsorption studies and development of a dispersive solid-phase extraction (d-SPE) protocol coupled with HPLC-UV.

Main Results:

  • Cell-Azo demonstrated successful grafting and light-induced reversible isomerization.
  • Maximum adsorption capacity was 19.86 mg·g⁻¹ at pH 9.0.
  • UV light triggered AMP release with 90.54% desorption efficiency, significantly higher than in the dark.
  • The d-SPE-HPLC-UV method showed wide linearity, low LOD (7 μg·L⁻¹), and good precision.
  • Recoveries in spiked urine samples ranged from 69.53-75.68%.

Conclusions:

  • Cell-Azo is an effective intelligent sorbent for light-driven solid-phase extraction of AMP.
  • The light-controlled release mechanism offers a significant advantage over conventional methods.
  • Cell-Azo presents a promising green and controllable alternative for trace AMP analysis in complex biological samples.