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1,3-Dicarbonyl Rhodamines for Live-Cell Single-Molecule Super-Resolution Imaging.

Qiang Peng1,2, Yangfan Wang2, Bei Zheng3

  • 1Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang Province, China.

Angewandte Chemie (International Ed. in English)
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed novel 1,3-dicarbonyl-rhodamine derivatives for super-resolution microscopy. These probes enable precise imaging of cellular structures and dynamics in live cells with high spatial and temporal resolution.

Keywords:
1,3‐Dicarbonyl compoundsrhodaminesuper‐resolution imaging

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Area of Science:

  • Chemical Biology
  • Microscopy
  • Cell Biology

Background:

  • 1,3-Dicarbonyl compounds possess versatile chemical properties applicable in various scientific fields.
  • Rhodamine fluorophores are widely used in biological imaging.
  • Single-molecule localization microscopy (SMLM) requires advanced probes for high-resolution live-cell imaging.

Purpose of the Study:

  • To synthesize and characterize novel 1,3-dicarbonyl-rhodamine derivatives.
  • To evaluate the suitability of these derivatives for single-molecule localization microscopy (SMLM).
  • To demonstrate the application of these probes for live-cell super-resolution imaging and dynamic tracking.

Main Methods:

  • Integration of 1,3-dicarbonyl scaffold with rhodamine fluorophores.
  • Utilizing thermodynamic and photochemical properties for sparse localization and controlled photooxidation.
  • Employing click chemistry, HaloTag labeling, and phospholipid-targeting for specific labeling.
  • Super-resolution imaging and dynamic tracking of cellular components in live cells.

Main Results:

  • Successful construction of 1,3-dicarbonyl-rhodamine derivatives with tunable properties.
  • Demonstrated sparse localization, tunable emitter density, and self-triggered photooxidation cascade.
  • Achieved super-resolution imaging and dynamic tracking of actin, endoplasmic reticulum, mitochondria, and plasma membrane.
  • Resolved filopodia dynamics with 22 nm spatial accuracy and 20 s temporal resolution over a 20-minute period.

Conclusions:

  • The developed 1,3-dicarbonyl-rhodamine derivatives are effective probes for SMLM.
  • These probes facilitate high-fidelity, long-term dynamic tracking of cellular structures in live cells.
  • The study showcases a novel approach for advancing super-resolution microscopy techniques.