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Using 3D Invasion properties of RCC Cell Lines In Vitro to predict their Metastatic Potential In Vivo.

Beatrice Cesana1, Laurie Nemoz-Billet2, Valentin Azemard1

  • 1Interdisciplinary Research Institute of Grenoble, IRIG-Biosanté, University Grenoble Alpes, CEA, INSERM UMR 1292, F-38000, Grenoble, France.

Cell Death Discovery
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

Three renal cell carcinoma (RCC) cell lines showed varied metastatic potential. Advanced 3D models accurately predicted this behavior, offering tools for better cancer outcome prediction and treatment strategies.

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Area of Science:

  • Oncology
  • Cancer Biology
  • Translational Research

Background:

  • Renal cell carcinoma (RCC) presents significant heterogeneity, complicating prognosis and treatment.
  • Accurate prediction of tumor aggressiveness and therapeutic response requires models mimicking cancer cells and their microenvironment.
  • Developing reliable in vitro models is crucial for advancing RCC treatment strategies.

Purpose of the Study:

  • To investigate the invasive and metastatic properties of distinct RCC cell lines (RCC10, RCC7, 786-O).
  • To evaluate the predictive accuracy of 3D spheroid invasion and patient-derived tumoroid systems for RCC metastatic behavior.
  • To establish reliable in vitro models for predicting RCC progression and therapeutic response.

Main Methods:

  • Utilized three RCC cell lines (RCC10, RCC7, 786-O) with distinct signaling profiles.
  • Assessed metastatic potential using in vitro and in vivo models, including zebrafish embryo extravasation, chorioallantoic membrane (CAM) assays, and mouse models.
  • Employed 3D spheroid invasion and patient-derived RCC tumoroid systems for comparative analysis.

Main Results:

  • RCC7 and 786-O cell lines demonstrated significantly higher metastatic potential compared to RCC10.
  • RCC7 exhibited partial epithelial-mesenchymal transition (pEMT) characteristics and upregulated key metastatic markers.
  • 3D spheroid invasion and tumoroid models accurately predicted the metastatic behavior and aggressiveness of RCC cell lines in vivo.

Conclusions:

  • 3D in vitro models, including spheroid invasion and tumoroid systems, can reliably predict RCC metastatic potential and aggressiveness.
  • These models offer valuable tools for assessing tumor outcome and guiding therapeutic strategies in renal cell carcinoma.
  • The findings underscore the utility of advanced 3D models in mimicking tumor microenvironment and predicting clinical behavior.