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In vitro evaluation of single application per session of two-bottle bleaching gels
Leticia Condolo1, Murilo Belo Teixeira2, Laryssa Mylenna Madruga Barbosa3
1Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil.
Journal of Dentistry
|February 28, 2026
Summary
Total Blanc One Step Drop (TD) showed lower hydrogen peroxide (HP) diffusion into the pulp chamber compared to other in-office bleaching gels. This difference is attributed to TD's initial neutral pH, making it a potentially safer option.
Area of Science:
- Dental Materials Science
- Biomaterials
- Restorative Dentistry
Background:
- In-office bleaching agents are widely used for aesthetic dental treatments.
- Understanding the behavior of hydrogen peroxide (HP) in dental bleaching is crucial for safety and efficacy.
- Two-bottle in-office bleaching systems require careful evaluation of their interaction with dental tissues.
Purpose of the Study:
- To evaluate hydrogen peroxide (HP) permeability into the pulp chamber.
- To assess the bleaching efficacy and physicochemical properties of various in-office bleaching gels.
- To analyze enamel surface effects, microhardness, and roughness after bleaching treatments.
Main Methods:
- Six different 35% HP in-office bleaching gels were applied in three 30-minute sessions.
- HP permeability was measured using UV-Vis spectrophotometry.
- Bleaching efficacy, pH, HP concentration, enamel morphology, microhardness, and roughness were analyzed.
Main Results:
- All gels showed significant bleaching efficacy and induced enamel surface alterations.
- Pola Office (PO) exhibited the highest HP diffusion, while Total Blanc One Step Drop (TD) showed the lowest.
- TD maintained a more neutral initial pH compared to other acidic gels, correlating with reduced HP diffusion.
Conclusions:
- A 30-minute application session is sufficient for bleaching efficacy and morphological changes with tested gels.
- Total Blanc One Step Drop (TD) demonstrated significantly lower HP diffusion into the pulp chamber due to its initial neutral pH.
- TD gel may offer a safer profile regarding pulp exposure while maintaining comparable bleaching outcomes.

