Polysaccharide from Pleurotus djamor: extraction optimization with response surface methodology, preliminary...

The purpose of this work was to optimise the hot water extraction process utilising response surface methodology with Box-Behnken design modelling of Pleurotus djamor polysaccharides (PDP). The ideal parameters of extraction were a ratio of solvent-to-solid of 10.15 mL/g, 1.65 h of time, and 82.08 °C of temperature. The PDP extraction yield of 6.09% was obtained by hot water extraction. According to a chemical component analysis, PDP included 69.6% total carbohydrates, 6.86% protein, and 6.17% uronic acid. PDP demonstrated strong in vitro antioxidant scavenging action for OH˙, Cu²⁺, Fe³⁺, β-carotene bleaching inhibition, and chelating ability. The PDP inhibited 53.09% of murine fibroblast (L929) and 57.58% of murine metastatic melanoma cells (B16-F10) at a concentration of 100 μg/mL and possesses an immunomodulatory effect by enhancing macrophage phagocytosis. These results offer a comprehensive understanding of the efficient extraction of PDP and their importance in considering their potential applications.

The online version contains supplementary material available at 10.1007/s10068-025-02085-6.