Evaluation of a Hydrodynamic Ozonation System for Gutta-Percha Disinfection and Structural Characterization
Jessica Sthefanny Carvalho Souza1,2, Pedro Augusto Laurindo Igreja Marrafa1,2,3, Maycon Crispim Oliveira de Carvalho1,2
1Postgraduate Program in Biomedical Engineering, Biomedical Engineering Institute, Universidade Anhembi Morumbi (UAM), São José dos Campos 12247-004, São Paulo, Brazil.
ACS Omega
|March 2, 2026
Summary
Ozonated water effectively disinfects gutta-percha cones, eliminating bacteria without damaging the material. This hydrodynamic system offers a safe, novel method for endodontic material sterilization.
Area of Science:
- Biomaterials Science
- Endodontics
- Microbiology
Background:
- Gutta-percha (GP) is crucial for endodontic obturation but prone to contamination.
- Current disinfection methods for GP have limitations, risking material damage.
- Sterilization challenges exist due to GP's thermoplastic properties.
Purpose of the Study:
- To assess the efficacy of a hydrodynamic ozonated water system for disinfecting GP cones.
- To evaluate the impact of ozonated water on GP material properties.
- To determine the safety and potential clinical applications of this disinfection method.
Main Methods:
- Experimentally contaminated GP cones were treated with flowing ozonated water (5 and 7 min).
- Microbiological analysis assessed bacterial inactivation (Enterococcus faecalis).
- Material characterization included FTIR, profilometry, SEM, and tensile strength testing.
Main Results:
- Complete inactivation of Enterococcus faecalis was achieved with 7-minute ozonated water exposure (57.2 mg/L).
- FTIR showed minimal changes; profilometry revealed reduced surface roughness.
- Tensile strength was preserved, with a decreased elastic modulus, suggesting improved adaptation.
Conclusions:
- Hydrodynamic ozonated water is a safe and effective disinfectant for endodontic materials.
- This method shows potential for clinical use in endodontic procedures.
- Ozonated water treatment preserves GP integrity while enhancing its properties.
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