Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nuclear blebs are composed of variable chromatin states but consistently enrich transcription initiation relative to elongation.

bioRxiv : the preprint server for biology·2026
Same author

Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrity.

Journal of cell science·2026
Same author

Topoisomerase I inhibition suppresses nuclear blebbing via RNA Pol II stalling and nuclear stiffening.

bioRxiv : the preprint server for biology·2026
Same author

CTCF maintains centromere function and mitotic fidelity.

Journal of cell science·2026
Same author

Lamin B loss in nuclear blebs is rupture dependent whereas increased DNA damage is rupture independent.

Journal of cell science·2025
Same author

High-temporal-resolution on-site multiplex biomarker monitoring in small animals using microfluidic digital ELISA.

Biosensors & bioelectronics·2025

Related Experiment Video

Updated: Mar 3, 2026

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
10:26

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

Published on: December 22, 2011

13.7K

Time-Lapse Into Immunofluorescence Imaging Using a Gridded Dish.

Nick Lang1, Catherine G Chu1, Andrew D Stephens1,2

  • 1Biology, University of Massachusetts Amherst, Amherst, MA, USA.

Bio-Protocol
|March 2, 2026
PubMed
Summary

Time-lapse into immunofluorescence (TL into IF) imaging now directly links live-cell dynamics with static immunofluorescence markers. This simplified method uses gridded dishes, eliminating the need for complex tracking software.

Keywords:
Cell biology dynamicsImmunofluorescenceLive-cell imagingMechanobiologyNuclear dynamicsNuclear ruptureStatic imagingTime-lapse imaging

More Related Videos

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

7.0K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.6K

Related Experiment Videos

Last Updated: Mar 3, 2026

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
10:26

Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix

Published on: December 22, 2011

13.7K
Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

7.0K
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
06:00

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

11.6K

Area of Science:

  • Cell Biology
  • Mechanobiology
  • Microscopy Techniques

Background:

  • Live-cell imaging provides dynamic cellular information, while immunofluorescence offers static molecular details.
  • Connecting these imaging modalities is crucial for understanding cellular processes like nuclear bleb formation but is often technically challenging.
  • Existing methods require specialized software or stage position tracking, hindering accessibility.

Purpose of the Study:

  • To develop a simplified protocol for directly correlating time-lapse live-cell imaging with subsequent immunofluorescence imaging.
  • To provide an accessible method for cell biologists to link cellular dynamics with molecular composition.
  • To overcome the limitations of current techniques in connecting live and static imaging.

Main Methods:

  • Cells were cultured and imaged using time-lapse microscopy on gridded imaging dishes.
  • The gridded coordinate system on the dish allowed for precise relocation of individual cells after live-cell imaging.
  • Standard immunofluorescence staining was performed on the same cells, guided by the established coordinates.

Main Results:

  • The protocol successfully enabled direct matching of cells between time-lapse and immunofluorescence images based on coordinates.
  • This method eliminated the need for third-party analysis software or complex stage position measurements.
  • The time-lapse into immunofluorescence (TL into IF) protocol proved straightforward and effective for mechanobiology research.

Conclusions:

  • Time-lapse into immunofluorescence (TL into IF) imaging provides a direct and accessible link between live-cell dynamics and static immunofluorescence.
  • The use of gridded imaging dishes is the key innovation, simplifying cell tracking and correlation.
  • This protocol enhances the ability of cell biologists to study dynamic cellular events and their molecular consequences.