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Related Experiment Video

Updated: Jul 3, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Amyloid and the Cross-Beta Architecture.

Kyle Morris¹, Karen E Marshall¹, Youssra Al-Hilaly¹

¹School of Life Sciences, University of Sussex.

Journal of Visualized Experiments : Jove

|March 2, 2026

Summary

This summary is machine-generated.

Amyloid fibrils, implicated in diseases like Alzheimer's, possess a repetitive structure. This study details methods to determine this architecture across various amyloid fibril sources.

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Last Updated: Jul 3, 2026

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Area of Science:

Structural biology
Biochemistry
Biophysics

Background:

Amyloid fibrils are misfolded protein assemblies with repetitive structures.
They are associated with diseases such as Alzheimer's and type 2 diabetes.
Functional roles in organisms include providing strength and scaffolding.

Purpose of the Study:

To describe methods for determining the underlying architecture of amyloid fibrils.
To highlight common features of amyloid fibrils from diverse sources.
To provide a framework for comparing experimental data with model structures.

Main Methods:

X-ray fiber diffraction was initially used to model amyloid structures.
Advanced diffraction analysis of oriented and semi-crystalline samples provides detailed architecture.
Methods cover sample preparation, data collection, and data analysis.

Main Results:

Detailed structural models of the generic cross-β structure have been developed.
Analysis reveals the organization of peptides into repetitive architectures.
Common features across pathogenic, functional, and synthetic amyloid fibrils are described.

Conclusions:

Understanding amyloid fibril architecture is crucial for studying protein misfolding diseases.
The described methods enable detailed structural characterization of diverse amyloid fibrils.
This work facilitates comparison between experimental data and theoretical models.