Related Experiment Video
Updated: Aug 16, 2026

09:31
Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
Published on: October 16, 2016
Synthesis by an established lymphocyte cell line from a rheumatoid synovium
Arthritis and Rheumatism
|April 1, 1978
Abstract:
Lymphocytes derived from the synovium of a patient with rheumatoid arthritis were shown to synthesize predominantly IgG as measured by an equilibrium binding assay. Chromatographic separation of the radiolabeled lymphocyte culture supernatant revealed rheumatoid factor activity associated with IgG and IgM. In addition, immunofluorescence studies on fixed lymphocytes demonstrated that the majority of cells stained positive for both IgG and rheumatoid factor.
Related Concept Videos
Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

