Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

1.5K
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Chemical and Biological Portable Mass Spectrometer (CB-pMS) with Automated Sampling and Hybrid Ionization for Online Detection of Bioaerosols and VOCs.

Analytical chemistry·2026
Same author

Associations of probiotics combined with Tongli Gongxia Chinese medicine with intestinal barrier biomarkers and day 7 short-chain fatty acids in patients with severe acute pancreatitis.

Frontiers in cellular and infection microbiology·2026
Same author

Dielectric levitation optical tweezers for powerful mesoscale biomanipulation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Enhancing All-Solid-State Batteries Performance Through Thickness Control and Surface Passivation of Thermally Evaporated Lithium Metal Anodes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

TRIM65 accelerates VSMC-derived foam cell formation and arteriosclerosis progression by inhibiting mitophagy.

Molecular and cellular biochemistry·2026
Same author

Drug-eluting bead TACE improves the efficacy of HAIC combined with molecular-targeted therapy and anti-PD-1 immunotherapy in large hepatocellular carcinoma with inferior vena cava and/or right atrium tumor thrombus: a multicenter retrospective cohort study.

BMC cancer·2026

Related Experiment Video

Updated: May 6, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

9.9K

Streamlined Digital Microfluidics-Mass Spectrometry Strategy for Extracellular Vesicle Enrichment and Lipid

Menglei Zhao1,2, Hang Li2,3,4, Yudan Ma3

  • 1Beijing Advanced Innovation Center for Intelligent Robots and Systems, School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Analytical Chemistry
|March 3, 2026
PubMed
Summary

A new microfluidic platform rapidly isolates extracellular vesicles (EVs) from small samples for lipid analysis. This method accelerates EV lipidomics, aiding research in immunometabolism and diagnostics.

More Related Videos

Extraction of Extracellular Vesicles from Whole Tissue
09:03

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

14.8K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

2.2K

Related Experiment Videos

Last Updated: May 6, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

9.9K
Extraction of Extracellular Vesicles from Whole Tissue
09:03

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

14.8K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

2.2K

Area of Science:

  • Biochemistry and Molecular Biology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Extracellular vesicles (EVs) are crucial for intercellular communication and implicated in diseases like cancer and immune regulation.
  • Current EV isolation methods are time-consuming, require large sample volumes, and hinder downstream lipidomic analysis, especially for scarce samples.
  • Studying lipid profiles of specific EV subtypes, like macrophage-derived EVs, is challenging with existing techniques.

Purpose of the Study:

  • To develop a rapid, low-volume method for isolating and performing on-chip lipidomic profiling of EVs using digital microfluidics (DMF) integrated with mass spectrometry (MS).
  • To enable efficient EV lipidomic analysis from trace biological samples, overcoming limitations of conventional methods.
  • To investigate subtype-dependent lipid remodeling in macrophage-derived EVs.

Main Methods:

  • Development of a multiplexed digital microfluidic (DMF) platform utilizing ZrO2-coated magnetic beads (ZrO2@Fe3O4) for EV capture via Lewis acid-base interactions with EV membrane phosphate groups.
  • On-chip lipid extraction and direct analysis by mass spectrometry (MS) following EV isolation.
  • Validation of the platform's speed, recovery rate, and lipid coverage using HeLa-cell-derived EVs and primary macrophage EVs.

Main Results:

  • The DMF-MS platform isolates EVs from microliter-scale samples in under 15 minutes with a 78% recovery rate, significantly faster than traditional ultracentrifugation (>2 hours).
  • The method preserves EV activity and provides broad lipid coverage, as demonstrated with HeLa-cell EVs.
  • Analysis of macrophage EVs revealed distinct lipid remodeling between M0 and M2 states, with M2 EVs showing increased anti-inflammatory fatty acids and decreased cholesterol esters.

Conclusions:

  • The integrated DMF-MS strategy offers a rapid, sensitive, and low-volume solution for EV isolation and lipidomic profiling.
  • This platform facilitates the study of rare EV populations and accelerates research in immunometabolism and diagnostics.
  • The ability to resolve subtype-specific lipid profiles in EVs opens new avenues for understanding cellular communication and disease mechanisms.