Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

5.5K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.5K
Western Blotting01:15

Western Blotting

14.3K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
14.3K
Antibody Structure and Classes01:25

Antibody Structure and Classes

7.7K
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
7.7K
Methods of Classification and Identification01:28

Methods of Classification and Identification

2.3K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Swiss-PO 2025: Advancing Cancer Mutation and Structural Analysis for Precision Oncology With the Latest Release.

JCO precision oncology·2026
Same author

Dual CD8 and TCR editing in regulatory T cells mediates HLA-A2-restricted tissue-specific homing.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

ModelCIF Update: Supporting Emerging Classes of Computational Macromolecular Models.

Journal of molecular biology·2026
Same author

Universal paratope-epitope interaction patterns in antibody-antigen structures.

Protein science : a publication of the Protein Society·2025
Same author

Hybrid quantum/classical docking of covalent and non-covalent ligands with Attracting Cavities.

Scientific reports·2025
Same author

A fingerprint approach to pioneer structure-based T cell receptor repertoire analysis and specificity prediction.

Frontiers in immunology·2025

Related Experiment Video

Updated: May 2, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

11.5K

Novel universal domain-centric method for protein classification.

Shakiba Fadaei1, Fanny S Krebs2, Vincent Zoete3,4

  • 1Computer-Aided Molecular Engineering, Department of Oncology UNIL-CHUV, Ludwig Institute for Cancer Research, University of Lausanne, Lausanne, Switzerland.

Scientific Reports
|March 3, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method using physico-chemical properties to classify human protein kinases, including previously unclassified ones. This machine learning approach enhances accuracy beyond traditional phylogenetic methods.

Keywords:
ClassificationDomainEnsemble learningKinaseMachine learning

More Related Videos

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
05:43

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Published on: January 24, 2017

7.6K
Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

6.8K

Related Experiment Videos

Last Updated: May 2, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
08:04

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

11.5K
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
05:43

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Published on: January 24, 2017

7.6K
Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
07:49

Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group

Published on: August 16, 2017

6.8K

Area of Science:

  • Biochemistry
  • Bioinformatics
  • Structural Biology

Background:

  • Human protein kinases form a large superfamily of ~500 genes, historically classified by phylogenetic relationships.
  • Many protein kinases remain unclassified, and traditional phylogeny-based methods using sequence alignments neglect residue physico-chemical properties.

Purpose of the Study:

  • To develop a novel classification method for human protein kinases by incorporating physico-chemical properties.
  • To classify previously unlabeled kinases and refine existing classifications.

Main Methods:

  • Utilized detailed physico-chemical descriptions of protein kinase sequences.
  • Employed an unsupervised classification method and machine learning techniques.
  • Identified class-specific structural regions for validation.

Main Results:

  • Successfully classified previously unlabeled human protein kinases.
  • Developed a novel approach that aligns with and refines existing phylogeny-based classifications.
  • Demonstrated the identification of class-specific structural regions.

Conclusions:

  • Physico-chemical properties offer deeper insights into protein kinase classification beyond sequence alignments.
  • The novel machine learning-based classification method enhances accuracy and can be applied to other protein families.