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Updated: Mar 6, 2026

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

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Measuring deiodinase activity: a need for standardization?

Joëlle Wiersema¹, Petra Mohácsik², Balázs Gereben²

¹Endocrine Laboratory, Department of Laboratory Medicine, Amsterdam UMC, Location AMC, University of Amsterdam, Amsterdam, The Netherlands.

European Thyroid Journal

|March 4, 2026

Summary

This summary is machine-generated.

Measuring deiodinase activity is key for thyroid hormone research. However, significant variations exist between labs, meaning results are only comparable within a single lab without standardized methods.

Keywords:

assay deiodinase radioactive tracers thyroid hormone metabolism

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Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

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Published on: May 10, 2015

Area of Science:

Endocrinology
Biochemistry
Molecular Biology

Background:

Thyroid hormones are crucial regulators of metabolism, produced by the thyroid gland and metabolized in peripheral tissues.
Deiodinases (types 1, 2, and 3) are key enzymes in thyroid hormone metabolism, differing in function and expression.
Accurate measurement of deiodinase activity is vital for thyroid hormone research.

Purpose of the Study:

To assess variability in deiodinase activity measurements across different laboratories.
To evaluate the comparability of deiodinase assay results between research institutions.
To highlight the need for quality assurance in deiodinase activity assays.

Main Methods:

A method comparison study was conducted involving five experienced laboratories measuring deiodinase activity.
Participating laboratories utilized various assays and protocols for deiodinase activity determination.
Data on deiodinase activity levels were collected and analyzed for inter-laboratory comparison.

Main Results:

Significant differences in determined deiodinase activity levels were observed between the participating laboratories.
Variations in measurement results could be attributed to differences in techniques and protocols used.
Absolute deiodinase activity values were generally not comparable across different laboratories.

Conclusions:

Direct comparison of absolute deiodinase activity is often limited to within-laboratory results.
External quality control is most effective when laboratories employ identical techniques.
Implementing internal quality controls is recommended for ensuring consistent and reliable deiodinase activity measurements over time.